What Bacteria Can Live In Alcohol?
Asked 281 days ago
Similar Questions for from Yahoo Answers|
Yes. Some viruses and bacteria can live for a long time. If you have a virus when you use it and don't sterilize it after, the virus will still be there.|
Think about it... Would you use the same glass over and over without washing it?
||How do I make alcohol from apple juice?
I am 21, but I want to try and make my own alcohol. I live at college, so what if I filled up a Poland Spring water bottle with the apple juice from the fountain at school and put yeast in? Would that work, and if so, how long would it take?
Here are instructions to make hard cider (fermented alcoholic apple juice):|
"doesn’t contain chemical preservatives" that could kill the yeast.
"pour your cider into the brewpot and simmer it over medium heat for about 45 minutes. This will kill most of the wild yeasts and bacteria in the cider."
"sanitized fermentation bucket — an unsanitized bucket may spoil the cider"
"Within a day or two you should see the airlock start to bubble. The gas it’s releasing is carbon dioxide, a byproduct of the fermentation process. Congratulations, your soft cider is on its way to becoming a delicious, inebriating elixir of the gods! This bubbling should subside within two weeks, signifying an end to the primary fermentation. After that, let the cider sit another week to allow the yeast to settle out"
And, some yeasts are better than others. Yeast used for wine is best.
The link has detailed instructions. Typically it is a few weeks to do it correctly.
Sterile Bacteriological media (Mueller-Hinton agar or Tryptic Soy Agar and tubes of Tryptic soy broth)
Cultures of Bacteria
Sterile physiological saline
Alcohol lamp, tweezers, and beaker of alcohol for flame sterilization of the tweezers.
Antibiotic disks (small paper disks impregnated with the antibiotic being studied).
A biologist to be your mentor
After selecting a bacterium to study, you will need to grow a stock culture of the bacterium usually about one full day. Using a sterile swab, seed the surface of the medium in a petri dish. You want to swab the plate three different directions so there is heavy uniform growth on the surface. Place one antibiotic disk in the center of the dish using flame-sterilized tweezers. To flame sterilize the tweezer, dip the tips of the tweezers into the 70% alcohol. Using the flame of the alcohol lamp, ignite the alcohol on the tweezers. Keep your hand pointed down or the alcohol can run onto your fingers and burn them!
Place the lid on the petri dish, label the bottom of the dish with a sharpie pen with date, organism, antibiotic used. Incubate the plate upside down for 24 hours. After incubation, check the plate for growth of bacteria. There should be heavy growth at the edges of the plate and may have a clear zone around the disk. If there is a clear zone look for colonies inside the clear zone. Any colony in the zone is antibiotic resistant. If there are no colonies, subculture the growth right at the edge of the clear zone. Repeat the procedure as many times as you have time to do so or until colonies grows in the clear zone!
All materials that have live bacteria on or in it, has to be disinfected. Swabs, dishes, and tubes should be soaked in disinfectant. Your work area should be wiped down with disinfectant before and after working.
You do need a trained biologist or microbiologist supervising your work. There are a number of things that can injure you.